**Please note: The BRMC Confocal Core is located in Longwood 221 EBRC 315.
Researcher Sam Lee trying out our new confocal microscope!
The facility is equipped with an Olympus FV1200 (without multi-photon capability) consisting of:
- Argon 458/477/488/514 nm laser
- Green HeNE 543 nm
- Red HeNe 633 nm laser
- Coherent ULTRA near infrared tunable Titanium Sapphire laser (690 – 1040 nm)
The system allows a range of experiments with fixed or live samples with time lapse function available on all systems making it possible to perform experiments over time courses in 5 different channels.
Other facility equipment includes the motorized upright microscope and a scanning module with 32 narrow wavelength band single channel detectors.
Imaging can be done with most standard fluorophoresin either single-photon. Software will be available for FRAP, DIC, ratiometric imaging, and complex time series experiments.
In addition to the confocal scanning system, the Core has metamorph 3-D reconstruction, and high-resolution transmitted light and epifluorescence imaging objective and a camera.
